TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG
SKU: BTL-TB-MB-00189 |
Brand: Transgen Biotek
Product Description
| Cat Number | AQ322-01 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 100 rxns×20 μl system |
| Description | TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination. Highlights • Use TransScript® II Multiplex Probe One-Step Enzyme Mix UDG, 2×PerfectStart® Multiplex Probe One-Step Reaction Mix to efficiently synthesize RNA into first-strand cDNA for qPCR. It is easy to operate and reduces the chance of contamination during operation. • Using UDG enzyme and dUTP to effectively prevent cross-contamination of PCR products with accurate data. • High sensitivity, high specificity and the data are accurate. Applications • Singleplex to 4-plex RT-qPCR detection. • High-copy and low-copy gene detection. • RNA templates with high GC content or complex secondary structure. • Detection of RNA virus or trace amounts of RNA. Storage Store at -20°C for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| Note | The product is for research use only |
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